Services
Downstream
Cultiply is a comprehensive platform for the development (CRO service) and production (CMO service) of a wide variety of microorganisms, proteins, enzymes and other biological components.
Centrifugation
Centrifugation is a widely used method for separating microbial biomass from the supernatant, although it also allows the fractionation of different cellular components. At Cultiply, we have laboratory, pilot and industrial scale equipment to carry out centrifugation processes.
Cell disruption
Cell disruption is a process used to release cellular contents. Some of its many functions include the recovery of proteins or other intracellular compounds, inactivating cultures, and yielding extracts from them. At Cultiply, we use reliable and economic disruption technology based on mechanical procedures, avoiding the use of chemicals and hydrolytic enzymes.
Filtration
At Cultiply, we use tangential flow filtration (TFF) as a process for increasing cell concentration or to fractionate or concentrate different cellular components including proteins, lipids and organelles. Tangential flow filtration is an economical, robust and scalable procedure with innumerable advantages over conventional filtration.
Freeze-drying
Freeze-drying is a downstream process that Cultiply uses as a method of preserving microorganisms and proteins, among other biological components. Its main advantage over other preservation procedures lies in its ability to treat sensitive products at high temperatures.
Spray-drying
The Spray-Drying technique is used as a system for drying and preserving biological products. It evaporates most of the moisture from a product and is a cost-effective and robust process. At Cultiply, we use this technology in the production of alternative proteins or biofertiliser products.
Protein purification
Protein purification comprises a series of techniques that separate one or more target proteins from a complex sample. Among the techniques employed are molecular exclusion chromatography, ion exchange, hydrophobic interaction and affinity. The right combination of these techniques enables the production of high purity proteins.
